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P302-05 Sigma-Aldrich

Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)

  •  NACRES NA.81

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Properties

Related Categories Cell Culture, Endothelial Cells, Primary Cell Culture, Primary Cells
Quality Level   100
biological source   Porcine pulmonary artery (Normal healthy)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 38
morphology   Endothelial
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cardiovascular diseases, diabetes
shipped in   dry ice
storage temp.   −196°C

Description

General description

PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).

Cell Line Origin

Artery

Application

cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs

Components

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Preparation Note

• 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PPAEC Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Pipette Controller from Brand
Protocols & Articles

Protocols

Protocol: Porcine Pulmonary Artery Endothelial Cells Culture

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