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P3032 Sigma-Aldrich

Penicillin G sodium salt

powder, BioReagent, suitable for cell culture

Synonym: Benzylpenicillin sodium salt

  • CAS Number 69-57-8

  • Empirical Formula (Hill Notation) C16H17N2NaO4S

  • Molecular Weight 356.37

  •  Beilstein Registry Number 3834217

  •  EC Number 200-710-2

  •  MDL number MFCD00069666

  •  eCl@ss 34010400

  •  PubChem Substance ID 24898384



Related Categories Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antibiotics by Mechanism of Action,
product line   BioReagent
form   powder
potency   ≥1477 units per mg
application(s)   cell culture | mammalian: suitable
solubility   H2O: 100 mg/mL (Solutions should be filter sterilized and stored at 2-8°C for 1 week or at -20°C for extended periods. Solutions are stable at 37°C for 3 days.)
suitability   suitable for cell culture
Mode of action   cell wall synthesis | interferes
antibiotic activity spectrum   Gram-positive bacteria



Penicillin G is a narrow spectrum antibiotic derived from Streptococcus pneumoniae. It has been used to study the diagnostic and therapeutic implications of gentamicin-resistant Enterococcus faecalis sequence type 6 with reduced penicillin susceptibility and in cell culture both alone and combined with streptomycin and other antibiotics.

Biochem/physiol Actions

Mode of Action: Penicillin G acts by inhibiting cell wall synthesis through binding to penicillin binding proteins (PBPs), inhibiting peptidoglycan chain cross-linking.

Antimicrobial spectrum: This product is active against gram-positive and gram-negative bacteria.


Solutions should be filter sterilized and stored at 2-8°C for 1 week or at -20°C for more lengthy periods. Solutions are stable at 37°C for 3 days. The sodium salt is soluble in H2O at 100 mg/mL.

General description

Chemical structure: ß-lactam

Other Notes


Keep container tightly closed in a dry and well-ventilated place.Product contains Penicillin Keep in a dry place.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Request a Serum Sample

Cell Culture Guide
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