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P3125 Sigma

Papain from papaya latex

buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein

Synonym: Papainase




Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.

Biochem/physiol Actions

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.


1 g in serum bottle

100, 250, 500 mg in serum bottle

Physical form

Suspension in 0.05 M sodium acetate, pH 4.5, containing 0.01% thymol

Price and Availability

Seppro Protein Depletion Technology

Biomedical Applications

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Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

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Enzymes & Proteins

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Peer-Reviewed Papers


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