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  • P3237 - Monoclonal Anti-phospho-PAK1 (pThr212) antibody produced in mouse

P3237 Sigma

Monoclonal Anti-phospho-PAK1 (pThr212) antibody produced in mouse

~2 mg/mL, clone PK-18, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, P-PA,
species reactivity   mouse, human, rat
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.2-0.4 μg/mL using a whole extract of transfected and activated COS-7 (monkey kidney) cells expressing phosphorylated PAK1
clone   PK-18, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 68 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PAK1(5058)
mouse ... Pak1(18479)
rat ... Pak1(29431)
biological source   mouse
conjugate   unconjugated



synthetic peptide corresponding to amino acids 203-217 (pThr212) of human PAK1.

General description

p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK. In the neurons, the reorganization of cytoskeleton during the neurite outgrowth is modulated by phosphorylation of PAK1 at Thr212 that regulates a specific signalling pathway
Monoclonal Anti-Phospho-PAK1 (pThr212) reacts specifically with human PAK1 phosphorylated at Thr212 (68 kDa), and does not detect the unphosphorylated epitope.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-phospho-PAK1 (pThr212) may be used for immunoblotting at a working concentration of 0.2-0.4 μg/mL. The antibody is also suitable for indirect ELISA, immunocytochemistry and protein microarray.

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Certificate of Analysis

Certificate of Origin

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Western Blot Protocol | Immunoblotting Protocol

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