• USA Home
  • P3288 - Phosphate buffer pH 7.2 at 25 °C

EMAIL THIS PAGE TO A FRIEND
P3288 Sigma-Aldrich

Phosphate buffer pH 7.2 at 25 °C

for hematology and histology staining techniques

Purchase

Description

General description

Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve the living tissues. It is also used in immunochemical staining procedures. Phosphate buffer serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4).

Application

Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.

Reconstitution

Each vial will prepare 3.8 L of buffer, 8.3 mM.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How to solubilize Product P3288, Phosphate Buffer pH 7.2?
The instructions for reconstitution of Product P3288, Phosphate buffer pH 7.2 at 25 °C areindicated on the Wright-Giemsa protocol (see attached packaging insert).Briefly, Product P3288, Phosphate buffer pH 7.2 should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Store Phosphate Buffer at room temperature (18-26°C). Store working phosphate buffer solution at 2-8°C. Discard the working phosphate solution if turbidity or visible bacterial growth is present.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?