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P3292 Sigma-Aldrich

Pancreatin from porcine pancreas

powder, suitable for cell culture, 4 × USP specifications

Synonym: Pancreatin from hog pancreas

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Culture, Cell Dissociation, Cell Dissociation (Cell Culture Tested),
form   powder
contains   lactose or sucrose as extender (The sucrose used might contain up to 3.25% starch.)
application(s)   cell culture | mammalian: suitable
Agency/Method   USP (specifications)
storage temp.   −20°C

Description

Packaging

25, 100 g in poly bottle

Application

Pancreatin from porcine pancreas has been used to assess the treatment of steatorrhea by lipase supplementation therapy, to investigate treatment options for pancreatic diabetes in patients experiencing the decompensated stage of chronic pancreatitis, and to safely and effectively remove formalin-fixed tissues from arterial grafts without leading to structural damage or loss in fiber integrity.

Biochem/physiol Actions

Pancreatin contains enzymatic components including trypsin, amylase and lipase, ribonuclease, and protease, produced by the exocrine cells of the porcine pancreas. This combination of enzymes allows it to hydrolyze proteins, starch and fats. Pancreatin will convert not less than 25 times its weight of potato starch into soluble carbohydrates in 5 minutes in water at 40°C, will digest not less than 25 times its weight of casein in 60 minutes at pH 7.5 at 40°C and will release not less than microequivalents of acid per min per mg pancreatin from olive oil at pH 9.0 at 37°C.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
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References

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