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P3391 Millipore

Protein A-Sepharose® from Staphylococcus aureus

lyophilized powder

Synonym: Protein A–Agarose

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Properties

Related Categories Affinity Chromatography, Affinity Resins, Antibodies, Antibody Purification, Antibody Purification and Characterization,
form   lyophilized powder
extent of labeling   3 mg per mL
matrix   Sepharose CL-4B
matrix activation   cyanogen bromide
matrix attachment   amino
matrix spacer   1 atom
capacity   ≥16 mg/mL binding capacity (human IgG)
storage temp.   2-8°C

Description

General description

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Application

Protein A-Sepharose was used:
• for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
• in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
• as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
• in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.

Protein A-Sepharose® is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Packaging

1, 1.5, 5 g in glass bottle

250 mg in glass bottle

Quantity

Swelling: 1 g swells to approx. 4 ml.

Physical form

Supplied as lyophilized powder stabilized with lactose and dextran.

Legal Information

Sepharose is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

How do I swellP3391, Protein A-Sepharose® resin?
The lyophilized product should be swollen in buffer A for 30 minutes or longer at room temperature. Do not stir with any kind of mechanical stirrer.One gram of powder typically swells to 3-4 mL of the hydrated gel.This, along with other useful information (such as the formulation of buffer A) can be found on the product information sheet (under Documents, above).
How can I store the hydrated P3391, Protein A-Sepharose® resin?
Suspensions stored in the refrigerator in the presence of a suitable bacteriostatic agent, such as 20% ETOH, retain IgG binding activity. Another storage option is to put the resin into phosphate buffer saline, pH 7 with about 0.01% preservative (thimerosal or sodium azide).
What can P3391, Protein A-Sepharose® resin tolerate?
Protein A Sepharose CL-4B exhibits high chemical and mechanical stability, and is stable over the pH range 2-11. It withstands high concentrations of denaturants, such as urea and guanidine hydrochloride. The gel displays high thermal stability but is not autoclavable. Furthermore, the gel can, if handled with care, be used many times.
How do I clean Product P3391, Protein A-Sepharose®, resin?
It may be possible to clean the resin by washing it with 10-20 volumes of 100 mM Tris or borate buffer, pH8.5, containing 0.5-2.0 M NaCl, followed by 10-20 volumes of 100 mM acetate buffer, pH 4.0, containing0.5-2.0 M NaCl. Re equilibrate the resin with 20 volumes of buffer A.
What is the binding capacity of Product P3391, Protein A-Sepharose®,resin for my immunoglobulins?
A table is available on the product information sheet (under Documents, above).
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

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Protocols

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Peer-Reviewed Papers
15

References

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