• USA Home
  • P3487 - Monoclonal Anti-PTEN antibody produced in mouse

P3487 Sigma

Monoclonal Anti-PTEN antibody produced in mouse

~1 mg/mL, purified immunoglobulin, clone PTN-18, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Lipids in Cell Signaling, PT-PY,
species reactivity   rat, mouse, human
application(s)   immunocytochemistry: suitable using 4% paraformaldehyde-0.5% Triton X-100 fixation
  indirect ELISA: suitable
  microarray: suitable
  western blot: 5-10 μg/mL using whole extract of rat brain
clone   PTN-18, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt ~55 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PTEN(5728)
mouse ... Pten(19211)
rat ... Pten(50557)
biological source   mouse
conjugate   unconjugated



synthetic peptide corresponding to the C-terminus of rat PTEN (amino acids 386-403).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.


The epitope recognized by the antibody resides within amino acids 386-403 of rat PTEN.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

esiRNA Knockdown Efficiency Tested by Western Blotting

A valuable measure of the knock-down potency of any RNAi experiment is the reduction in protein level. Displayed below are the knock-down rates of  selected esiRNAs using quantitative western blot an...
Keywords: Cell proliferation, Gene expression, PAGE, Reductions, Transfection, Western blot


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot


Keywords: Angiogenesis, Cancer, Eliminations, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?