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  • P3492 - Monoclonal Anti-phospho-Progesterone Receptor (pSer190) antibody produced in mouse

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P3492 Sigma-Aldrich

Monoclonal Anti-phospho-Progesterone Receptor (pSer190) antibody produced in mouse

~1 mg/mL, clone 1154, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to Transcription Factors, Cell Biology,
conjugate   unconjugated
clone   1154, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using T47D breast carcinoma cells
  immunoprecipitation (IP): 2 μg/mL using protein lysate 1 mg
  western blot: 1 μg/mL using T47D breast carcinoma cells
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P06401
Gene Information   human ... PGR(5241)

Description

Immunogen

synthetic serine-phosphopeptide corresponding to amino acids 288-300 of human progesterone receptor (hPR).

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 0.08% sodium azide.

Safety & Documentation

Safety Information

WGK Germany 
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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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