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P3752 Sigma-Aldrich

Phosphatase, Acid from potato

lyophilized powder, ≥0.5 unit/mg solid

Synonym: (Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

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Description

Application

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, product P3752, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages.

Packaging

1 g in poly bottle

100 mg in poly bottle

Biochem/physiol Actions

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Protocols

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

3.2. Unit Definition – One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per minute at pH 4.8 at 37°C.
Keywords: Extinction coefficient

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Peer-Reviewed Papers
15

References

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