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P3775 Sigma

Anti-Protein A antibody produced in rabbit

fractionated antiserum, lyophilized powder



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biological source   rabbit
antibody form   fractionated antiserum
clone   polyclonal
form   lyophilized powder
packaging   vial of 2 mL lyophilized antiserum
application(s)   indirect ELISA: 1:100,000
conjugate   unconjugated
storage temp.   2-8°C



Protein A from Staphylococcus aureus


Reconstitute with 2 ml deionized water.

Physical form

Lyophilized from 0.01M phosphate buffered saline, pH 7.2


Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

General description

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

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Safety Information

NONH for all modes of transport
WGK Germany 
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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P6031 Protein A from Staphylococcus aureus, Soluble, essentially salt-free, lyophilized powder, extracellular

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