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P3775 Sigma-Aldrich

Anti-Protein A antibody produced in rabbit

fractionated antiserum, lyophilized powder



Related Categories Alphabetical Index, Antibodies, Miscellaneous, PR-PR, Primary Antibodies More...
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect ELISA: 1:100,000
form   lyophilized powder
storage temp.   2-8°C
packaging   vial of 2 mL lyophilized antiserum
antibody form   fractionated antiserum
Quality Level   200
antibody product type   primary antibodies


General description

Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.


Protein A from Staphylococcus aureus


Anti-Protein A antibody produced in rabbit has been used in immunoblotting.

Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

Physical form

Lyophilized from 0.01M phosphate buffered saline, pH 7.2


Reconstitute with 2 ml deionized water.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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P6031 Protein A from Staphylococcus aureus, Soluble, essentially salt-free, lyophilized powder, extracellular

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