• USA Home
  • P3813 - Phosphate buffered saline

P3813 Sigma-Aldrich

Phosphate buffered saline

powder, pH 7.4, for preparing 1 L solutions

Synonym: PBS

  •  MDL number MFCD00131855

  •  eCl@ss 32129211

  •  NACRES NA.25




• Phosphate buffered saline has been used in the washing solutions during immunofluorescence procedure.
• It has been used for the suspension of bacterial cells.
• It has been used during the fixation and immunostaining procedures.


Foil pouches


Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

IsProduct P3813, Phosphate buffered saline, a 1 X PBS solution?
Product P3813 when prepared as directed yields a 1 X PBS solution.
Does Product P3813, Phosphate buffered saline, contain calcium or magnesium ions?
There is no calcium nor magnesium added to the powder.
How is Product P3813, Phosphate buffered saline, supplied?
As a package of 10 foil pouches.
What is the shelf life of Product P3813, Phosphate buffered saline?
The quality for unopened foil pouches stored at room temperature is guaranteed for one year from the time of receipt. The product is expected to be stable for at least 5 years.
How long cansolutions of Product P3813, Phosphate buffered saline,be kept once prepared?
Sigma Aldrich has not evaluated the solution stability, but sterile solutions of PBS stored at 2-8°C can be expected to be stable for several months.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How much is in each pouch of Product P3813, Phosphate buffered saline?
Each pouch of phosphate buffered saline contains 8 g sodium chloride (e.g. Product No. S9625), 0.2 g potassium phosphate, monobasic (e.g. Product No. P5379), 1.15 g sodium phosphate, dibasic (e.g. Product No. S0876), 0.2 g potassium chloride (e.g. Product No. P4504), giving a total 9.55 g per packet.This is the standard PBS formulation.
Does Product P3813, Phosphate buffered saline, contain calcium, magnesium or sodium bicarbonate?
The formulation for this phosphate buffered saline product does not contain calcium, magnesium, or sodium bicarbonate.Sigma does not assay the product for the presence of these compounds (as impurities). None of these compounds are used in the method of preparation.
Is Product P3813, Phosphate buffered saline, sterile?
Phosphate buffered saline product number P3813 is not necessarily sterile.
Is Product P3813, Phosphate buffered saline, 1X or 10X?
Each pouch of phosphate buffered saline product number P3813 can be used to prepare 1 Liter of a 1X PBS.
Is Product P3813, Phosphate buffered saline, tested for RNase or DNase impurities?
Phosphate buffered saline product number P3813 is not tested for RNase or DNase impurities.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


HDM-PAMPA with the MultiScreen Permeability Filter Plate

Assays that predict passive absorption of orally administered drugs have become increasingly important in the drug discovery process. As previously described by Faller1 and Kansy2 such assays provide...
Keywords: Absorption, Drug discovery, Evaporation, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Ultraviolet-Visible spectroscopy

Immunofluorescence Labeling of Cells

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Microscopy, Solvents, Titrations

Immunohistochemistry Protocol

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

Membrane Integrity test for Lipid-PAMPA Artificial Membranes

The Parallel Artificial Membrane Permeation Assays (PAMPA) is a non-cell based assay designed to predict passive, transcellular permeability of drugs in early drug discovery. The assay is carried out...
Keywords: Drug discovery

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?