• USA Home
  • P3818 - Protein Disulfide Isomerase from bovine liver

P3818 Sigma

Protein Disulfide Isomerase from bovine liver

>95% (SDS-PAGE), ≥100 units/mg protein, lyophilized powder

Synonym: PDI




0.1 mg in poly bottle

250 μg in glass bottle

Package size based on protein content.

Unit Definition

One unit cause a change in A650 of 0.01 per min of a 1.0 mg/mL solution of insulin in the presence of dithiothreitol at pH 7.5 at 25 °C.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.


Protein Disulfide Isomerase(PDI) is a ubiquitous, highly conserved redox chaperone enzyme from the thioredoxin superfamily. It is mainly located in the ER, where it assists in protein-folding and thiol-disulfide exchanges. It is used to study functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi.

Biochem/physiol Actions

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.

Physical properties

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

Price and Availability

Expore Enzymes, Proteins & Cofactors

Celebrate Pi Day!
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is ...
BioFiles 2011, 6.4, 22.
Keywords: Anti-inflammatory agents, Apoptosis, Cancer, Cell signaling, Cellular processes, Gene expression, Indicators, Metabolism, Oxidations, Respiratory

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?