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  • P3902 - Anti-Pyk2 antibody produced in rabbit

P3902 Sigma

Anti-Pyk2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CAK-β, Anti-Proline rich Kinase 2



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Non-Receptor Tyrosine Kinase Antibodies,
species reactivity   mouse, rat, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections of human cerebellum.
  immunoprecipitation (IP): 3-5 μg/mL using 150-200 μg of PC-12 rat pheochromocytoma RIPA lysate
  microarray: suitable
  western blot: 1:2,000 using a whole extract of LPS-stimulated P388 mouse monocyte-macrophage cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 110-116 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PTK2B(2185)
mouse ... Ptk2b(19229)
rat ... Ptk2b(50646)
biological source   rabbit
conjugate   unconjugated



Synthetic peptide corresponding to amino acid residues 991-1009 of human Pyk2, coupled to KLH. This sequence is highly conserved in rat and mouse (1 amino acid substitution).

General description

Pyk2 (proline-rich kinase 2) is a protein belongs to Tyr protein kinase family and expresses mainly in tissues and cells like mesenchymal, epithelial, endothelial cells, neonatal cardiomyocytes, osteoclasts and neuronal cells. It has a crucial role in T and B cell antigen receptor signaling, cell cycle progression, metastasis, NK cytotoxicity, modulation of ion channel function and neuronal short- and long- term responses. Anti-Pyk2 antibody can be used to identify Pyk2 by immunohistochemistry. Rabbit anti-Pyk2 antibody reacts specifically with Pyk2 of rat, mouse and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide


Anti-Pyk2 antibody can be used in immunoprecipitation (110-116 kD) and western blotting (diluted 1:2000) using of LPS-stimulated P388 mouse monocyte-macrophage cell extract. It can also be used in microarray.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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