• USA Home
  • P4056 - Platelet-Derived Growth Factor-BB from rat

EMAIL THIS PAGE TO A FRIEND
P4056 Sigma-Aldrich

Platelet-Derived Growth Factor-BB from rat

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: PDGF-BB

Purchase

Properties

Related Categories Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture,
Quality Level   100
biological source   rat
recombinant   expressed in E. coli
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
potency   1.62 ng/mL EC50
quality   endotoxin tested
mol wt   ~30 kDa
packaging   pkg of 5x50 μg
  pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
application(s)   cell culture | mammalian: suitable
impurities   ≤1 EU/μg
UniProt accession no.   P31240
storage temp.   −20°C
Gene Information   rat ... Pdgfb(24628)

Description

General description

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Application

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

Other Notes

Dimer of the B chain of rat PDGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Request a Serum Sample

Pipette Controller from Brand
Protocols & Articles

Articles

Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.1 PDGF elicits multifunctional actions with a variety of cells, i...
Jennifer Fries
BioFiles 2009, 4.5, 20.
Keywords: Atomic absorption spectroscopy, Growth factors, Purification, Type

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?