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P4112 Sigma-Aldrich

Anti-phospho-PKB (pSer473) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-phospho-Akt

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Properties

Related Categories AKT Antibodies, AKT1 Antibodies, Alphabetical Index, Antibodies, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   microarray: suitable
  western blot: 1:1,000 using a whole extract of PDGF-treated mouse fibroblast NIH3T3 cells and a whole extract of H2O2-treated rat fibroblast Rat-1 cells
species reactivity   mouse, rat
mol wt   antigen 56 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P47196
Gene Information   human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)

Description

General description

Protein kinase B (PKB, also known as Akt) is a serine/threonine protein kinase and a key regulator of signal transduction processes. Important processes such as cell cycle, proliferation and apoptosis are regulated by PKB in response to stimuli such as growth factors, insulin and cytokines. Three isoforms of PKB have been identified, α, β and γ. PKB α is reportedly overexpressed in breast cancer cell lines, whereas PKB β is overexpressed in ovarian and pancreatic cancers. On cell stimulation by growth factors or insulin, PKB α translocates to the cell membrane, where it undergoes full activation by phosphorylation at Thr308 and Ser 473, by PDK1 and PDK2. PKB α plays a crucial role in several downstream pathways involved in glycogen synthesis, protein translation and cell proliferation. PKB is also a potent inhibitor of apoptotic cell death by suppressing BAD-induced death

Specificity

Anti-phospho PKB (pSer473) recognises PKB phosphorylated at serine 473 (56 kDa).

Immunogen

Synthetic phosphorylated peptide corresponding to (pSer473) PKBα (human, amino acids 461-477 with N-terminally added lysine), conjugated to KLH. The sequence is identical in rat, mouse, viral Akt and bovine PKBα and is highly conserved in chicken PKBα-(single amino acid substitution). The immunogen sequence is conversed to a lesser degree in PKBγ (88%) and PKBβ (64%).

Application

Anti-phospho-PKB (pSer473) antibody produced in rabbit has been used in western blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Alzheimer's Disease

Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions. Hallmark pathohistological findings of AD include widespread ...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 4–8
Keywords: Alzheimer Disease, Gene expression, Genetic, Genetics, Inflammation, Metabolism, Phosphorylations

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

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