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P4417 Sigma-Aldrich

Phosphate buffered saline

tablet

Synonym: PBS

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Description

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Application

Phosphate buffered saline has been used-
• for the production of platinum nanoparticles (NP-Pt) hydrocolloids
• for the washing of blood sample and SEM (scanning electron microscope) stubs prior to SEM (scanning electron microscopy)
• for preparing the solution of fluorescent lectin wheat germ agglutinin (WGA)
• for the dilution of capture antibody during ELISA (enzyme linked immunosorbent assay)
• for suspension of cell pellets during centrifugation

Packaging

50, 100 tablets in poly bottle

Reconstitution

One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Please Enter a Lot Number

Certificate of Origin

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Does Product P4417, Phosphate buffered saline (PBS), contain calcium or magnesium?
No.The complete formulation is given in the product listing.No calcium or magnesium salts are included in the preparation.
Doesone tablet ofProduct P4417, Phosphate buffered saline (PBS),make up 1X or 10X PBS?
When 1 tablet is reconstituted in 200 ml of water, the resulting solution is 1X PBS.
Can Product P4417, Phosphate buffered saline (PBS), solution be autoclaved?
Yes.PBS is not harmed by standard autoclave procedures.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

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Protocols

Antibody Purification using Protein A, Protein G, or Protein L Agarose

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Buffers, Cell culture, Dialysis, Filtration, Purification, Separation

ELISA Procedures

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Capture ELISA, Cell culture, Cell signaling, Enzyme-linked immunosorbent assay, Hormones, Indirect ELISA, Titrations

Immunofluorescence Labeling of Cells

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Microscopy, Solvents, Titrations

Immunohistochemistry Procedure

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Digestions, Solvents

Immunoprecipitation Procedure

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Buffers, Cell disruption, Centrifugation, Enzyme activity, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, PAGE, Size-exclusion chromatography, Western blot

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Peer-Reviewed Papers
15

References

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