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P4506 Sigma-Aldrich

Phosphodiesterase I from Crotalus atrox (Western Diamondback Rattlesnake)

Type IV, crude dried venom, ≥0.01 unit/mg solid

Synonym: 5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase

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Description

Application

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. Phosphodiesterase I, product P4506 from western diamondback rattlesnake, is used in phosphodiesterase activation assays for calmodulin. It is added to hydrolyze AMP. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates.

Phosphodiesterase I from Crotalus atrox (Western Diamondback Rattlesnake) has been used to analyse its effect on intracellular calcium concentration of neutrophils. It has also been used in combination with other enzymes to determine the activity of phosphatase enzyme.

Biochem/physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

Unit Definition

One unit will hydrolyze 1.0 μmole of bis(p-nitrophenyl) phosphate per min at pH 8.8 at 37 °C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SZ5999500

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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