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P4600 Sigma-Aldrich

ReadyMix Taq PCR Reaction Mix

with MgCl2

Purchase

Properties

Related Categories Core Bioreagents, Life Science Reagents for PCR, Molecular Biology, PCR Enzymes, PCR/Amplification,
form   liquid
feature   hotstart: no
concentration   1.5 units/reaction (50 μL reaction volume)
color   colorless
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

General description

ReadyMix Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.

Application

For routine PCR amplifications

Features and Benefits

• Amplifies targets up to 7 kb in length

• ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

• ReadyMix format reduces setup time.

• Reduced risk of contamination from multiple pipet steps.

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL

Supplied with a vial of PCR grade water for dilution.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

History of PCR Discoveries - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Amplification, Clinical, Degradations, Detection methods, Diagnostic, Diseases, Forensic, Gene expression, Genetic, Molecular biology, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Separation, Transcription

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Protocols

PCR Protocol | Standard PCR Protocol

These steps are presented below in greater detail along with materials and reagent selection tips. This is a basic PCR protocol using Taq DNA polymerase.
Keywords: AGE, Amplification, Electrophoresis, Evaporation, Gel electrophoresis, PAGE, Polymerase chain reaction, Sequencing

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

PCR Master Mix

Sometimes known as super mix or ready mix, PCR master mix is a batch mixture of PCR reagents at optimal concentrations that can be prepared once and divided among many PCR tubes or 96-well PCR plates...
Keywords: Buffers, Gene expression, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
Keywords: Building blocks, Mass spectrometry, Organic synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Vitamins

Peer-Reviewed Papers
15

References

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