• USA Home
  • P4716 - Pectinase from Aspergillus niger

EMAIL THIS PAGE TO A FRIEND
P4716 Sigma-Aldrich

Pectinase from Aspergillus niger

Green Alternative

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

Synonym: Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger

Purchase

Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
Quality Level   200
product line   BioReagent
form   aqueous glycerol solution
greener alternative product characteristics   Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
application(s)   cell culture | plant: suitable
greener alternative category   Enabling
storage temp.   2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Application

Petctinase from Aspergillus niger has been used:
• for protoplasts liberation
• in the protoplast enzyme mixture for enzyme digestion
•  to conduct partial saccharification of sugars
• to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Packaging

25000, 100000 units in glass bottle

Biochem/physiol Actions

Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

Physical form

Solution in 40% glycerol

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Protocols

Enzymatic Assay of Pectinase

3.2 Unit Definition = = One unit will liberate 1.0 micromole of galacturonic acid from poly- galacturonic acid per hour at pH 4.0 at 25ºC.
Keywords: Enzyme activity, Enzymology, Oxidations

Related Content

Enzyme Explorer Key Resources: Lysing Enzymes

Cell Lysis Enzymes – How to tear down the Wall Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your...
Keywords: Amplification, Biochemistry, Cell culture, Cell disruption, Culture media, Enzymology, Metabolites, Molecular biology, PAGE, Purification, Sample preparations, Sequencing

Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Life Science Materials

With a broad portfolio of widely-cited reagents and kits supported by industry-leading expertise, we help you get the most out of your life science research.

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?