• USA Home
  • P4744 - Phosphatase substrate

EMAIL THIS PAGE TO A FRIEND
P4744 Sigma-Aldrich

Phosphatase substrate

powder

Synonym: 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

Purchase

Properties

Related Categories p-Nitrophenyl Phosphate Substrate Systems, Alkaline Phosphatase, Alphabetical Listing, Biochemicals and Reagents, Colorimetric,
Quality Level   200
form   powder
solubility   water: 100 mg/mL, clear to slightly hazy
storage temp.   −20°C
SMILES string   O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI   1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key   KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

Application

Phosphatase substrate has been used as a substrate in tartrate-resistant acid phosphatase (TRAP) assay and alkaline phosphatase (ALP).

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Packaging

1, 5, 10, 25, 50 g in glass bottle

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Protocols

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?