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P4780 Sigma-Aldrich

TWEEN® 80

suitable for cell culture, suitable for insect cell culture, viscous liquid

Synonym: POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

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Properties

Related Categories Biochemicals and Reagents, Biological Detergents, Cell Biology, Cell Culture, Cell Lysis Reagents,
description   non-ionic
form   viscous liquid
mol wt   ~1310
  average mol wt 1310
composition   Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)
CMC   0.012 mM
density   1.06 g/mL at 25 °C (lit.)
  1.07 g/cm3 at 25 °C (lit.)
suitability   suitable for cell culture
  suitable for insect cell culture

Description

General description

TWEEN 80 is a polyethylene sorbitol ester and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.

Application

TWEEN® 80 has been used:
• in Listeria monocytogenes enumeration using filtration method
• as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion
• as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

Packaging

1 gal in poly bottle

100, 500 mL in poly bottle

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
WG2932500
Flash Point(F) 
>235.4 °F
Flash Point(C) 
>113 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

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