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P4780 Sigma-Aldrich

TWEEN® 80

suitable for cell culture, suitable for insect cell culture, viscous liquid

Synonym: POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

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Properties

Related Categories Biochemicals and Reagents, Biological Detergents, Cell Biology, Cell Culture, Cell Lysis Reagents,
Quality Level   200
description   non-ionic
form   viscous liquid
mol wt   average mol wt ~1310
composition   Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
CMC   0.012 mM
density   1.06 g/mL at 25 °C (lit.)
  1.07 g/cm3 at 25 °C (lit.)
storage temp.   room temp
InChI   1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+
InChI key   RGPBUVUVZKQNHD-MDZDMXLPSA-N

Description

General description

TWEEN 80 is a polyethylene sorbitol ester and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

TWEEN® 80 has been used:
• in Listeria monocytogenes enumeration using filtration method
• as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion
• as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing

Packaging

1 gal in poly bottle

100, 500 mL in poly bottle

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
WG2932500
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Related Content

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Peer-Reviewed Papers
15

References

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