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P4978 Sigma-Aldrich

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Synonym: CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Alkaline Phosphatase Enzymes, Analytical and Industrial Enzymes, Biochemicals and Reagents,
Quality Level   200
grade   for molecular biology
form   buffered aqueous glycerol solution
mol wt   ~80 kDa
concentration   ≥10,000 units/mL
foreign activity   DNase, RNase, none detected
shipped in   wet ice
storage temp.   −20°C
Gene Information   cow ... ALPI(280993)

Description

General description

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Application

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Components

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Other Notes

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Alkaline Phosphatase, Calf Intestinal (CIP)

Product Description Product Summary Storage buffer Procedure: Dephosphorylation of DNA Heat Inactivation Materials Reference
Keywords: Cloning, Peptide synthesis, Precipitation, Purification

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay.
Keywords: Enzyme activity, Extinction coefficient, PAGE

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Peer-Reviewed Papers
15

References

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substrate

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