• USA Home
  • P5368 - Phosphate buffered saline

EMAIL THIS PAGE TO A FRIEND
P5368 Sigma-Aldrich

Phosphate buffered saline

BioPerformance Certified, pH 7.4

Synonym: PBS

Purchase

Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffer Convenience Packaging,
grade   BioPerformance Certified
  for molecular biology
impurities   BioBurden, tested
  endotoxin, tested
pH   7.4
cation traces   Fe: ≤10 ppm
  heavy metals (as Pb): ≤5 ppm
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase, RNase, Protease, none detected

Description

Packaging

Foil Pouch

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Does Product P5368, Phosphate buffered saline (PBS), contain calcium and magnesium?
No.The complete formulation is shown in the product listing.When reconstituted, the resulting solution is 10 mM phosphate buffer, with 138 mM sodium chloride and 2.7 mM potassium chloride.
If reconstituted with sterile water, will Product P5368, Phosphate buffered saline (PBS), be sterile?
No.To make this PBS sterile, the solution should be either sterile-filtered or autoclaved.
What volume ofProduct P5368, Phosphate buffered saline (PBS),does each pack make?
When the contents of a pouch is dissolved in 1 Liter of water, it makes 1X PBS, pH 7.4 at 25°C.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?