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P5459 Sigma

Protease from Bacillus licheniformis

7-15 units/mg protein (biuret), aqueous propylene glycol solution (containing sodium benzoate and potassium sorbate)

Synonym: Proteinase from Bacillus licheniformis, Subtilisin A



Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,
form   aqueous propylene glycol solution (containing sodium benzoate and potassium sorbate)
concentration   ≥50 mg/mL
solubility   0.85% sodium chloride: soluble, clear (Diluted)
  H2O: miscible
storage temp.   2-8°C



5 g in glass bottle

Package size based on protein content.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.


Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.

The enzyme from Sigma has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

General description

The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.

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Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Personal Protective Equipment 
UN 3082 9 / PGIII
WGK Germany 
Protocols & Articles


Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis.

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


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