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P5493 Sigma-Aldrich

Phosphate buffered saline

10× concentrate, BioPerformance Certified, suitable for cell culture

Synonym: PBS




Phosphate buffered saline has been used:
• as a cell wash solution in human dental pulp stem cells (DPSCs)
• for perfusion studies in dissected brain samples
• as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling
• as wash solution for ovary and sperm during in vitro culture and fertilization


4L package size comes in a box with a spigot for spill free dispensing.

Other Notes

When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Does Product P5493, Phosphate buffered saline (PBS), contain calcium and magnesium?
No, it does not contain calcium and magnesium.
What is the concentration of the 10X stock solution of Product P5493, Phosphate buffered saline (PBS)?
As formulated, the 10X solution is 100 mM phosphate and 1.54 M sodium chloride.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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