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P5936 Sigma-Aldrich

Pectolyase from Aspergillus japonicus

Green Alternative

suitable for plant cell culture, lyophilized powder

Synonym: PEL1

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Biochemicals and Reagents, Chemical Synthesis, Complex Carbohydrate Analysis,
Quality Level   200
form   lyophilized powder
activity   ≥0.3 unit/mg solid
greener alternative product characteristics   Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
application(s)   cell culture | plant: suitable
Featured Industry   Agriculture
greener alternative category   Enabling
storage temp.   2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.

Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Packaging

1 g in poly bottle

100, 250 mg in poly bottle

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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