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P5975 Millipore

Amino-terminal Met-FLAG-BAP Fusion Protein

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Properties

Related Categories FLAG Control Proteins, FLAG System, Molecular Biology, Proteomics, Purification and Detection,
mol wt   ~49 kDa
shipped in   dry ice
storage temp.   −20°C

Description

Application

Amino-terminal Met-FLAG-BAP Fusion Protein is useful for assurance of the functional integrity of ANTI-FLAGÒ M2 and M5 monoclonal antibodies in immunological procedures such as Western blotting, ELISA, immunoprecipitation, fluorescence microscopy, light microscopy and FACS.

Biochem/physiol Actions

Amino-terminal Met-FLAG-BAP control protein is a 468 amino acid N-terminal Met-FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular weight of 49.4 kDa. The Amino-terminal Met-FLAG-BAP control protein migrates as a 45-55 kDa band by SDS-PAGE depending on the electrophoretic conditions.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
HG1933000
Protocols & Articles

Protocols

Immunoprecipitation of FLAG fusion proteins using monoclonal antibody affinity gels

Immunoprecipitation (IP) can be used for efficient, high-yield isolation and purification of proteins fused to the FLAG® peptide tag. IP is performed with the ANTI-FLAG® M2 affinity gel, which is a h...
Keywords: Cell disruption, Centrifugation, Electrophoresis, Gene expression, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Size-exclusion chromatography, Tagged proteins

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