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P6407 Sigma-Aldrich

Poly-D-lysine hydrobromide

mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Synonym: PDL

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Properties

Related Categories Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,
InChI Key   MEXAGTSTSPYCEP-UHFFFAOYSA-N
sterility   γ-irradiated
product line   BioReagent
form   lyophilized powder
mol wt   70,000-150,000
packaging   pkg of 10 X 5 mg
  pkg of 5 mg
solubility   H2O: soluble 50 mg/mL, clear, colorless
suitability   suitable for cell culture
storage temp.   −20°C

Description

Packaging

5, 10×5 mg in glass bottle

Application

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Components

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

This product has a molecular weight of 70,000-150,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Do you have a protocol for coating cell cultureware or glass coverslips/slides with Product P6407, Poly-D-Lysine?
The protocol for coating surfaces with poly-D-lysine is the same as for poly-L-lysine. The optimal conditions must be determined for each cell line and application. In general, the following steps can be used.1. Add 50 ml of sterile tissue culture grade water to 5 mg of poly-D-lysine.2. Aseptically coat culture surface with 0.5-1.0 ml of solution per 25 cm2. Rock gently to ensure even coating of the culture surface.3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.4. Allow to dry at least two hours before introducing cells and medium.
Does Product P6407, Poly-D-lysine hydrobromide, need to be dialyzed to remove the hydrogen bromide?
The hydrogen bromide will not interfere with the cell growth. The hydrogen bromide salts are used to make our poly-L-lysine solutions that are cell culture tested. For these products, there is no interference in cell growth from the bromide.
Why would I use Product P6407, Poly-D-lysine hydrobromide, instead of poly-L-lysine?
Certain cells can digest poly-L-lysine, in which case the poly-D-lysine should be used.
How do I determine which molecular weight range of poly-lysine to use?
Poly-lysine is used to coat surfaces to increase the charge present. The lower molecular weight poly-L-lysine (30-70 kD) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kD) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kD. Please check the literature for information on the molecular weight range used for your cell type.
How should I store solutions of poly-lysine?
Poly-lysine solutions (0.1 to 1 mg/ml) can be sterile-filtered (0.2 um) and should be stored at 2-8 °C. Solutions should not be stored frozen as thawing can cause a decrease in the size (molecular weight range) of the polymer.
What protocol should I follow for coating Transwells with Product P6407, Poly-D-lysine dihydrochloride?
The same protocol can be followed for coating plates with poly-lysine. The transwell membrane is immersed in the poly-lysine solution.
How long can I store poly-lysine coated slides or plates?
Plates and/or slides can be stored after drying, protected from dust for up to 1 year. Inspect the plates/slides to make sure there are no cracks in the coating prior to use. Each lab should determine the maximum length of time for storage based on the application.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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References

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