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P7181 Sigma-Aldrich

Polyethylene glycol solution

Hybri-Max, 50 % (w/v), average mol wt 1,450, sterile-filtered, BioReagent, suitable for hybridoma

Synonym: PEG solution

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Properties

Related Categories Cell Culture, Conditioned media supplements and reagents, Essential Chemicals, Hybridoma Reagents, Materials Science,
Quality Level   200
grade   Hybri-Max
sterility   sterile-filtered
product line   BioReagent
form   solution
mol wt   average mol wt 1,450
concentration   50 % (w/v)
application(s)   cell culture | hybridoma: suitable
impurities   endotoxin, tested
storage temp.   2-8°C
SMILES string   C(CO)O
InChI   1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key   LYCAIKOWRPUZTN-UHFFFAOYSA-N

Description

General description

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Application

Polyethylene glycol solution has been used in the hybridization of the following:
• irradiated camel fibroblast cells with A23 hamster cells
• mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
• rabbit splenocytes and cell line 240E-1

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Biochem/physiol Actions

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

Packaging

Packaged in sealed ampules under nitrogen.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN2810 - class 6.1 - PG 3 - EHS - Toxic, liquids, organic, n.o.s., HI: all
WGK Germany 
WGK 3
Flash Point(F) 
212.0 °F - closed cup - Not applicable
Flash Point(C) 
100 °C - closed cup - Not applicable

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Protocols & Articles

Articles

Polyethylene Glycol (PEG) Selection Guide

Poly(ethylene glycol) (PEG) is a synthetic, hydrophilic, biocompatible polymer with widespread use in biomedical and other applications. PEGs are synthesized using a ring-opening polymerization of et...
Keywords: Adhesion, Bacterial conjugations, Click chemistry, Molecular biology, PEGylations, Polymerization reactions, Ring-opening polymerization

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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Peer-Reviewed Papers
15

References

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