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P7367 Sigma-Aldrich

PNGase F from Elizabethkingia meningoseptica

BioReagent, ≥95% (SDS-PAGE), for proteomics

Synonym: N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase

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Properties

Related Categories 3.5.x.x Acting on C-N other than peptides, 3.x.x.x Hydrolases, Antibody Characterization and Analysis, Antibody Glycan Analysis, Biochemicals and Reagents,
Quality Level   200
grade   for proteomics
product line   BioReagent
assay   ≥95% (SDS-PAGE)
form   powder
shelf life   ≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml)
  ≥1 yr at 2‑8 °C
  Solution is stable for at least 3 freeze-thaw cycles
mol wt   ~36 kDa
concentration   ≥300 units/mL
  ≥50 units/mL
optimum pH   ~8.6
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Empowering biochemists to analyze glycoproteins with exceptional reproducibility

Protein glycosylation is one of the most common and structurally diverse types of protein post translational modifications (PTM). The particular pattern of glycosylation as well as the location of th...
Keywords: Apoptosis, Chromatography, Glycosylations, Growth factors, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Normal-phase chromatography, Post translational modifications, Separation, Solid phase extractions

N-Linked Glycan Strategies

Use of the endoglycosidic enzymes such as PNGase F (N-Glycosidase F) is the most effective method of removing virtually all N-linked oligosaccharides from glycoproteins. A variety of endoglycosidases...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Detergents, Digestions, Mass spectrometry, Nucleic acid denaturation, Proteomics

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Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers
15

References

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