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P7457 Sigma-Aldrich

Carboxy-terminal FLAG-BAP Fusion Protein



Related Categories FLAG Control Proteins, FLAG System, Molecular Biology, Proteomics, Purification and Detection,
form   (Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2)
mol wt   ~49 kDa
shipped in   dry ice
storage temp.   −20°C


General description

Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).


Carboxy-terminal FLAG-BAP Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).

Biochem/physiol Actions

The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.

Other Notes

Control protein

Preparation Note

Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml

Legal Information

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Anti-FLAG® M2 Magnetic Beads

Anti-FLAG® M2 magnetic beads provide an easy, fast and convenient method for the detection and capture of fusion proteins with the FLAG® peptide sequence. Beads are composed of a murine derived, anti...


Immunoprecipitation of FLAG fusion proteins using monoclonal antibody affinity gels

Immunoprecipitation (IP) can be used for efficient, high-yield isolation and purification of proteins fused to the FLAG® peptide tag. IP is performed with the ANTI-FLAG® M2 affinity gel, which is a h...
Keywords: Cell disruption, Centrifugation, Electrophoresis, Gene expression, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Size-exclusion chromatography, Tagged proteins

Peer-Reviewed Papers


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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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