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  • P7496 - Anti-Protein Disulfide Isomerase antibody produced in rabbit

P7496 Sigma-Aldrich

Anti-Protein Disulfide Isomerase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Erp58, Anti-PDI



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoplasm,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 10-20 μg using RIPA lysate from rat NRK cells
  indirect immunofluorescence: 1:250 using human HeLa cells
  western blot: 1:2,000 using whole extract of mouse NIH3T3 cells
species reactivity   bovine, mouse, rat, human
mol wt   antigen 57 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P07237
Gene Information   human ... P4HB(5034)
mouse ... P4hb(18453)
rat ... P4hb(25506)


General description

Prolyl 4-hydroxylase subunit β (P4HB) or protein disulfide isomerase is a redox-regulated thiol-containing protein. The gene encoding this protein is localized on human chromosome 17q25.3.


protein disulfide isomerase purified from bovine liver.


Anti-Protein Disulfide Isomerase antibody produced in rabbit has been used in immunoblotting, immunoprecipitation and immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Prolyl 4-hydroxylase subunit β (P4HB) or protein disulfide isomerase acts as a molecular chaperone in the endoplasmic reticulum of cells and also as an oxidoreductase. It associates with steroid hormones and modulates their actions, concentrations and storage. P4HB accelerates the formation of disulphide bonds in proteins and hence aids in their folding.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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