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P7626 Sigma

Phenylmethanesulfonyl fluoride

≥98.5% (GC)

Synonym: α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 5, 25, 100 g in glass bottle

250 mg in glass bottle

Application

Inhibits serine proteases such as trypsin and chymotrypsin. Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase. Not as effective or as toxic as DFP. Effective concentration 0.1-1 mM. Half-life = 1 hr. at pH 7.5.

Phenylmethanesulfonyl fluoride has been used in following applications:
• cell fractionation.
• used as a supplement in nuclear protein extraction.
• inhibitor of cholesterol esterase (CE) and pseudocholinesterase (PCE).
• used for the collection of blood prior to centrifugation to quantify plasma ANP levels.

Biochem/physiol Actions

Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.

Price and Availability


LC-MS Grade Solvents and Reagents

Ultrafree Centrifugal Filter Units

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2928 8(6.1) / PGII
WGK Germany 
3
RTECS 
XT8040000

Frequently Asked Questions

How should Product P7626, Phenylmethanesulfonyl fluoride (PMSF), solutions be prepared?
Stock solutions of 100-200 mM can be prepared in anhydrous isopropanol or anhydrous absolute ethanol. PMSF is unstable in the presence of water. This information can be found on the Product Information sheet by clicking on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/p7626pis.Par.0001.File.tmp/p7626pis.pdf
How should stock solutions of Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), be stored?
A 200 mM solution in dry solvent is stable for at least 9 months when stored at 2-8°C. This information can be found on the Product Information sheet by clicking on the following link: http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p7626pis.pdf
What does Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), inhibit?
PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases. This information can be found on the Product Information sheet by clicking on the following link:http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p7626pis.pdf
What is the effective amount of Product P7626,  Phenylmethanesulfonyl fluoride (PMSF), to be used?
The effective concentration range is 0.1-1 mM. A final concentration of 5 mM was used for proteinase K inhibition.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

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This technical article is a compilation of select fluorescence-based activity assays developed by Roar Biomedical now offered through Sigma-Aldrich in kit format. The methods are well-characterized, ...
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Keywords: Addition reactions, Cardiovascular, Chromatography, Clinical, Detection methods, Drug discovery, Esterifications, Gas chromatography, High performance liquid chromatography, Separation, Thin layer chromatography

Protocols

MTP Activity Assay Kit Protocol

The kit is sufficient for 100 assays in 200 µL total assay volume. Donor Particle                                0.4 mL     Catalog Number MAK110A Acceptor Particle                            0.4 mL ...
Keywords: Buffers, Homogenization, Sample preparations

Peer-Reviewed Papers
15

References

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