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  • P7634 - 3-Phosphoglyceric Phosphokinase from baker's yeast (S. cerevisiae)

P7634 Sigma-Aldrich

3-Phosphoglyceric Phosphokinase from baker's yeast (S. cerevisiae)

ammonium sulfate suspension, ≥1000 units/mg protein

Synonym: 3-Phosphoglycerate kinase, ATP:3-Phospho-D-glycerate 1-phosphotransferase



Related Categories 2.7.x.x Phosphorus containing groups, 2.x.x.x Transferases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   ammonium sulfate suspension
concentration   1.0-10.0 mg/mL
foreign activity   Glyceraldehyde-3-phosphate dehydrogenase ≤0.1%
shipped in   wet ice
storage temp.   2-8°C



3-Phosphoglyceric Phosphokinase generates ATP by catalyzing the transfer of a phosphate group from 1,3-diphosphoglycerate to ADP. 3-Phosphoglycerate Phosphokinase is used to study glycolysis and gluconeogenesis. It has also been used to study low molecular weight GTP-binding proteins and mechanisms of inhibition of glyceraldehyde-3-phosphate dehydrogenase.

The enzyme has been used in the assay of glyceraldehyde-3-phosphate dehydrogenase.

Biochem/physiol Actions

3-Phosphoglyceric Phosphokinase catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP to generate ATP and 3-phosphoglycerate. 3-Phosphoglycerate Phosphokinase activity is essential for glycolysis and gluconeogenesis.

Unit Definition

One unit will convert 1.0 μmole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 °C.

Physical form

Crystalline suspension in 3.0 M (NH4)2SO4 and 0.04 M tetrasodium pyrophosphate solution, pH 8.0

Analysis Note

Protein determined by Modified Warburg-Christian.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin

Protocols & Articles


Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

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Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers


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