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P7643 Sigma-Aldrich

PIPES dipotassium salt

≥99% (titration)

Synonym: 1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt

  • CAS Number 108321-27-3

  • Empirical Formula (Hill Notation) C8H16K2N2O6S2

  • Molecular Weight 378.55

  •  MDL number MFCD00069751

  •  PubChem Substance ID 24898738

  •  NACRES NA.25

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers
Quality Level   200
assay   ≥99% (titration)
form   crystalline powder
useful pH range   6.1 - 7.5
pKa (25 °C)   6.8
solubility   water: 0.5 g/mL, clear, colorless
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   [K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
InChI   1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2
InChI key   BJQYFCAMUXGZFN-UHFFFAOYSA-L

Description

General description

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

Application

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

Packaging

1 kg in poly bottle

25, 100, 500 g in poly bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Buffers - From Bnech to Bulk

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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