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  • P7899 - Anti-Peroxidase antibody produced in rabbit

P7899 Sigma-Aldrich

Anti-Peroxidase antibody produced in rabbit

fractionated antiserum, lyophilized powder



Related Categories Alphabetical Index, Antibodies, Miscellaneous, PD-PG, Plant Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect ELISA: 1:100,000-1:200,000
species reactivity   horseradish
form   lyophilized powder
storage temp.   2-8°C
antibody form   fractionated antiserum
Quality Level   200
antibody product type   primary antibodies


General description

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.

Peroxidase is a hemoprotein.

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.


Purified peroxidase from horseradish


Anti-Peroxidase antibody produced in rabbit has been used in:
• immunoblot assays
• enzyme-linked-immunosorbent assay (ELISA)
• immunoprecipitation

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2


Reconstitute with deionized water.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.

Analysis Note

Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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