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P7962 Sigma-Aldrich

Monoclonal Anti-PSTAIR antibody produced in mouse

clone PSTAIR, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Cyclins and CDKs,
conjugate   unconjugated
clone   PSTAIR, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  western blot: 1:4,000 using a COS-7 cell extract
species reactivity   human, lily, Xenopus, eel, Japanese quail, monkey, goldfish, onion, carp, mouse, starfish, amago salmon, ciliates
mol wt   antigen 33-34 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
Gene Information   human ... CDC2(983)
mouse ... Cdc2a(12534)


General description

In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in complex formation with cyclins. The availability of monoclonal antibody reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.

Monoclonal Anti-PSTAIR (mouse IgG1 isotype) is derived from the PSTAIR hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Cyclin-dependent kinase 1 (CDC2/CDK1) belongs to the Ser/Thr protein kinase family. It is located on human chromosome 10q21.


synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA.


Monoclonal Anti-PSTAIR antibody produced in mouse has been used in:
• western blot analysis
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation

Mouse monoclonal clone PSTAIR anti-PSTAIR antibody may be used for the localization of cyclin-dependent kinases containing the PSTAIR motif using various immunochemical assays including ELISA, immunoblot and immunoprecipitation.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Cyclin-dependent kinase 1 (CDC2/CDK1) controls various mitotic processes including the reorganization of the cytoskeleton, chromosome segregation, and formation and separation of daughter cells. It is essential for the multiplication of mammalian cell. It participates in tumorigenesis.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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