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P8038 Sigma

Proteinase, bacterial

Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

Synonym: Alkaline Protease, Protease from Bacillus licheniformis, Proteinase from Bacillus licheniformis, Subtilo peptidase A



Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.


1 g in glass bottle

50, 100, 250 mg in glass bottle


Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).


The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.

Biochem/physiol Actions

Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Price and Availability

Biomedical Applications
Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Personal Protective Equipment 
UN 3082 9 / PGIII
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
What is the pH optimum for Product P8038, Proteinase, bacterial?
Two references cite pH 7.5 as optimal for this enzyme: (a) Ricks et al., Biotechnol. Prog., 8, 197-203 (1992), (b) Doswald et al., Bioorg. Med. Chem.,  2, 403-410 (1994).
How long can I store stock solutions of Product P8038, Proteinase, bacterial?
Our notes indicate that a 0.1% solution (1 mg/mL) in PBS or TBS can be stored for about a week at 0-4°C.  For longer term storage, we would advise freezing the stock solution in aliquots.
What is the pI of Product P8038, Proteinase, bacterial?
One reference gives a pI of 9.4 for subtilisin Carlsberg. See: Zacharis, E. et al., Proc. Nat. Acad. Sci. USA, 96, 1201-1205 (1999).  
What is the molecular weight (MW) of Product P8038, Proteinase, bacterial?
The enzyme has a MW of 27.5-27.6 kDa. See DeLange & Smith, J. Biol. Chem., 243(9), 2134-2142 (1968).
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles


Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis.

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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
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