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P8047 Sigma-Aldrich

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment−R-Phycoerythrin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Anti-Immunoglobulins, Antibodies, HU-HZ,
conjugate   phycoerythrin (R-PE) conjugate
clone   polyclonal
biological source   goat
application(s)   direct immunofluorescence: 1:32
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   secondary antibodies

Description

General description

IgG is a glycoprotein antibody that contains two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4.

Immunogen

Purified human IgG

Application

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment R-Phycoerythrin antibody produced in goat has been used in bead-based assay and immunoassay multiplex magpix

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment-R-Phycoerythrin antibody is suitable for use in flow cytometry . The antibody can also be used for direct immunofluorescence (1:32).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.1 mM EDTA, 1 mM iodoacetamide, 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

0.25, 0.5 mL in glass insert

Biochem/physiol Actions

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), comprises the H chain constant region.

IgG antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . The use of anti-human IgG (γ-chain specific), F(ab′)2 fragment-R-Phycoerythrin antibody helps avoid background staining due to the presence of Fc receptors. The product is specific for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ, and Bence Jones λ myeloma proteins.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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