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P8169 Sigma-Aldrich

Phytagel

suitable for plant cell culture, BioReagent, powder

Synonym: Agar substitute gelling agent, Gellan Gum

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Properties

Related Categories Gelling Agents, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
product line   BioReagent
form   powder
application(s)   cell culture | plant: suitable
Featured Industry   Agriculture

Description

Application

Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.

Phytagel has been used:
• in the solidification of culture medium for root assays in Arabidopsis thaliana
• as a component of Solid Paul′s medium for Agrobacterium culture
• as a component of root induction medium in Ilex dumosa tissue culture

Packaging

1, 5 kg in poly drum

100, 250, 500 g in poly bottle

Other Notes

An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.

Reconstitution

To prevent clumping, add slowly to rapidly stirring medium prior to heating.

Legal Information

Phytagel is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Gelling Agents - Plant Tissue Culture Protocol

Agar has long been used to solidify media for plant tissue culture. The type of agar or gelling agent used can influence the growth of the tissue in culture. Both purity and cost of the gelling agent...
Keywords: Cell culture, Centrifugation, Immobilization, Inductively coupled plasma, Reductions

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Peer-Reviewed Papers
15

References

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