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P8287 Sigma-Aldrich

o-Phenylenediamine dihydrochloride

tablet, 10 mg substrate per tablet

  • CAS Number 615-28-1

  • Linear Formula C6H4(NH2)2·2HCl

  • Molecular Weight 181.06

  •  Beilstein/REAXYS Number 3912045

  •  MDL number MFCD00012966

  •  PubChem Substance ID 24898741

  •  NACRES NA.83

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Description

Application

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
RIDADR 
UN 1673 6.1 / PGIII
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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