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P8498 Sigma-Aldrich

Monoclonal Anti-Profilin antibody produced in mouse

~2 mg/mL, clone 2-11H7 (MAbPRF12a), purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, PR-PR, Plant Biology, Primary Antibodies More...
species reactivity   Arabidopsis thaliana
application(s)   immunohistochemistry: suitable
  indirect ELISA: suitable
  western blot: 1-2 μg/mL using extract of Arabidopsis sprout
clone   2-11H7 (MAbPRF12a), monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~14 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   Arabidopsis thaliana ... PFN2(829056), PRF1(816495), PRF3(835761)
biological source   mouse
antibody product type   primary antibodies
conjugate   unconjugated
UniProt accession no.   P14222



PRF1 protein.

General description

Profilins are highly conserved pan-allergens that occur in plant foods, pollen and latex. Profilins have five distinct isoforms (PRF1-5) that bind with actin filaments to regulate cytoskeletal organization. PRF1, 2 and 3 are constitutive profilins that occur in all vegetative cells. During the early stages of microsporogenesis, these isoforms are also expressed in microspores and ovules . Monoclonal Anti-Profilin antibody is specific for Arabidopsis constitutive profilin (PRF1 and PRF2 and weakly with PRF3). The antibody also reacts with tobacco profilin (approx. 14 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Monoclonal Anti-Profilin antibody is suitable for use in immunohistochemistry, western blot (1-2 μg/mL using extract of Arabidopsis sprout) and indirect ELISA.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
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Peer-Reviewed Papers


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P8248 Monoclonal Anti-Profilin antibody produced in mouse, ~2 mg/mL, clone 2B8 (MAbPRF45a), purified immunoglobulin, buffered aqueous solution

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