• USA Home
  • P8833 - Puromycin dihydrochloride from Streptomyces alboniger

EMAIL THIS PAGE TO A FRIEND
P8833 Sigma

Puromycin dihydrochloride from Streptomyces alboniger

powder, BioReagent, suitable for cell culture

Synonym: 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

  • CAS Number 58-58-2

  • Empirical Formula (Hill Notation) C22H29N7O5 · 2HCl

  • Molecular Weight 544.43

  •  Beilstein Registry Number 3853613

  •  EC Number 200-387-8

  •  MDL number MFCD00012691

  •  PubChem Substance ID 24898984

Purchase

Properties

Related Categories Antibacterial, Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
Quality Level   PREMIUM
biological source   Streptomyces alboniger
InChI Key   MKSVFGKWZLUTTO-FZFAUISWSA-N
product line   BioReagent
assay   ≥98% (HPLC)
form   powder
mol wt   mol wt 544.43 g/mol
packaging   pkg of 10 mg
  pkg of 100 mg
  pkg of 25 mg
color   yellow-white
solubility   H2O: soluble 50 mg/mL, clear, colorless to faintly yellow
suitability   suitable for cell culture
Featured Industry   Agriculture
Mode of action   protein synthesis | interferes
UniProt accession no.   P13249
storage temp.   −20°C

Description

Biochem/physiol Actions

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thus, functions as an acceptor substrate. Second, it competes with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center.

Caution

As supplied, this product remains active for four years when stored at -20°C.

Packaging

10, 25, 100 mg in glass bottle

Preparation Note

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

Application

Puromycin dihydrochloride from Streptomyces alboniger has been used:
• in the preparation of puromycin stock solution for puromycin sensitivity assay.
• to select lentivirus-infected cells containing puromycin resistant selection marker.

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

General description

Chemical structure: peptidyl nucleoside

Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger. It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
AU7355000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What are the approximate concentrations of Product P8833, Puromycin dihydrochloride, suitable for HeLa human uterus,B16 mouse melanoma and PC1.0 hamster adenocarcinoma cells?
HeLa human uterus: 3 μg/mL; B16 mouse melanoma: 1-3 μg/mL; PC1.0 hamster adenocarcinoma: 10 μg/mL.
What are the approximate concentrations of Product P8833, Puromycin dihydrochloride, suitable for HEK293, A431 and Jurkat cells?
HEK293: 2-3 μg/mL; A431: 1 μg/mL; Jurkat: 4 μg/mL.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Cell Culture Guide
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere wi...
BioFiles 2006, 1.4, 17.
Keywords: Antibiotics, Environmental, Peptide synthesis

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Plant Tissue Culture Products | Agriculture

Our combination of quality products, services and global business capabilities help you to maintain reagent consistency while ensuring suitability. Sigma-Aldrich allows you to standardize reagents ac...
Keywords: Antibiotics, Cell culture, Culture media, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Vitamins, transformation

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?