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P8859 Sigma-Aldrich

Anti-Protein Phosphatase X/C Interior antibody produced in sheep

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, PR-PR,
conjugate   unconjugated
clone   polyclonal
biological source   sheep
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human, bovine, mouse, rat
mol wt   antigen ~34 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P60510
Gene Information   human ... PPP4C(5531)
mouse ... Ppp2ca(56420)
rat ... Ppp4c(171366)

Description

General description

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. Protein Phosphatase X/C (or PP4) is a member of PP2A subfamily and shares 65% structural idENTITY_TYPE_CODE with PP2A in humans. PPX/C is actively involved in regulation of growth and organization of microtubule at the centrososmes and centrosome maturation during cell division by mitosis and meiosis. PPX/C reportedly has a pro-apoptotic role in T lymphocytes. PPX/C takes part in important cellular processes through signaling pathways involving NF-κB and c-Jun N-terminal kinase
Anti-Protein Phosphatase X/C Interior recognizes an internal sequence of protein phosphatase XCatalytic domain (34 kDa).

Immunogen

highly purified peptide corresponding to amino acid residues 289-302 of protein phosphatase X/C.

Application

Anti-Protein Phosphatase X/C is suitable for immunoblotting at a working dilution of 1:500-1:1000. The recommended dilution for immunocytochemistry is 1:500-1:1000.

Physical form

Solution supplied at 1 mg/mL in phosphate buffered saline with 0.08% sodium azide.

Safety & Documentation

Safety Information

WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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