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P9155 Sigma-Aldrich

Poly-L-lysine hydrobromide

mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Synonym: PDL HBr

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Properties

Related Categories Amino Acids, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Culture,
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
mol wt   30,000-70,000
packaging   pkg of 5 mg
solubility   H2O: soluble 50 mg/mL, clear, colorless
suitability   suitable for cell culture
storage temp.   −20°C

Description

General description

Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.

Application

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates. It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Cancer Stem Cells: New Targets for Cancer Therapy

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Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
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Poly-L-Lysine Cell Attachment Protocol

From our library of Articles, Sigma-Aldrich presents Poly-L-Lysine Cell Attachment Protocol
George Sitterley
BioFiles 2008, 3.8, 12.

Poly-lysine

Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are depend...
George Sitterley
BioFiles 2008, 3.8, 12.
Keywords: Adhesion, Cell culture, Enzyme-linked immunosorbent assay

Related Content

Bio Brief: Poly-L-lysine Hydrobromide [VIDEO]

Poly-L-lysine hydrobromide is a non-specific attachment factor useful for promoting cell adhesion to solid substrates, such as coverslips, slides, and microwell plates. Poly-L-lysine is a positively-...
Keywords: Adhesion

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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Peer-Reviewed Papers
15

References

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