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P9156 Sigma-Aldrich

Plasminogen from bovine plasma

lyophilized powder, ≥2.0 units/mg protein

Synonym: Profibrinolysin

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Properties

Related Categories Application Index, Biochemicals and Reagents, Blood, Cell Biology, Cell Signaling and Neuroscience,
Quality Level   200
biological source   bovine plasma
assay   2-7% protein basis (biuret)
form   lyophilized powder
composition   Protein, ~5% biuret
application(s)   histology: suitable
suitability   not suitable for streptokinase determination by clot formation procedure
storage temp.   2-8°C
Gene Information   cow ... PLG(280897)
human ... PLG(5340)

Description

Biochem/physiol Actions

The activated form of plasminogen, plasmin, was shown to promote the migration of melanoma and lung tumor cells plated on the extracellular matrix protein laminin by altering protein kinase C signaling.

Plasminogen is the inactive precursor of the protease plasmin. Plasminogen is activated by the action of either tissue plasminogen activator (tPA), which primarily activates the fibrinolytic (thrombolytic) activity of plasmin, or urokinase plasminogen activator (uPA), which is associated with extracellular matrix remodeling and cell migration. Plasmin cleaves fibrin/fibrinogen and blood coagulation factors V/Va and VIII/VIIIa. It activates matrix metalloproteinases by cleaving the inactive proenzymes. It is also involved in the activation of some growth factors, such as vascular endothelial growth factor (VEGF) and transforming growth factor β (TGF-β).

Other Notes

ε-Aminocaproic acid free

Unit Definition

One unit will produce a ΔA275 of 1.0 from α-casein in 20 min at pH 7.5 at 37 °C, when measuring perchloric acid soluble products in a volume of 5.0 mL. Activity determined after activation to plasmin with urokinase.

Physical form

Lyophilized powder containing NaCl, EDTA, lysine, and Tris buffer salts

Reconstitution

Solutions should be stored in buffer containing 20 mM lysine at neutral pH. The protein should be as concentrated as possible. Frozen in aliquots at −20 °C, it should be stable for several weeks.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Peer-Reviewed Papers
15

References

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