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  • P9249 - Monoclonal Anti-p53−Biotin antibody produced in mouse

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P9249 Sigma-Aldrich

Monoclonal Anti-p53−Biotin antibody produced in mouse

~1 mg/mL, clone DO-1, ascites fluid, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   biotin conjugate
clone   DO-1, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: 5-10 μg/mL using human A431
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 0.5-1 μg/mL using human A431 cell extracts
species reactivity   human
mol wt   antigen ~53 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P04637
Gene Information   human ... TP53(7157)

Description

General description

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Immunogen

recombinant human wild type p53. The epitope resides beween amino acids 20-25 of human p53.

Application

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree”freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

p53: DNA Damage Response and Cancer

p53 is a transcription factor that binds to defined consensus sites within DNA. A major function of p53 is to repress genes involved in cell growth stimulation while activating alternate genes involv...
Keywords: Acetylations, Adhesion, Apoptosis, Cancer, Carcinogens, DNA replication, Degradations, Eliminations, Environmental, Gene expression, Phosphorylations, Substitutions, Transcription

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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