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P9529 Sigma-Aldrich

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain

15-30 units/mg protein (in the presence of 0.03 mM Ca2+ and a saturating level (10 units per ml) of calmodulin (P2277)), lyophilized powder

Synonym: 3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience,
Quality Level   200
form   lyophilized powder
specific activity   1-5 units/mg protein (without added calmodulin)
  15-30 units/mg protein (in the presence of 0.03 mM Ca2+ and a saturating level (10 units per ml) of calmodulin (P2277))
mol wt   ~60 kDa
composition   Protein, 4.0-6.0% Lowry
foreign activity   2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%
  5′-ATPase ≤0.2%
  inorganic pyrophosphatase ≤0.8%
storage temp.   −20°C
Gene Information   cow ... PDE1A(281969)

Description

General description

Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca2+ second-messenger systems.

Application

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Packaging

Package size based on activated units

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Physical form

Lyophilized powder containing Tris-HCl buffer salts and lactose

Preparation Note

Affinity purified preparation which has been depleted of calmodulin.

Reconstitution

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4 °C.

Analysis Note

Protein determined by Warburg-Christian

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers
15

References

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