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PCR9601 Sigma-Aldrich

GenElute 96 Well PCR Clean-Up Kit

1 x 96 well plate, sufficient for 1 96-well plate purifications

Synonym: High Throughput Purification, PCR Purification, PCR Purification Kit, PCR Purification

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Properties

usage   sufficient for 1 96-well plate purifications
quality   1 x 96 well plate
storage temp.   room temp

Description

General description

GenElute 96 Well PCR Clean-Up Kit allows for high throughput purification of PCR products. The kit provides the necessary reagents for purification of highly pure PCR products. The DNA recovery is 75-90% for fragments of 100 to 10,000 bp and removes primers, primer-dimers, nucleotides, salts and polymerase.

Application

The purified PCR product is ready for a variety of downstream applications including sequencing, restriction digest, ligation and microarray analysis.

Features and Benefits

• Complete removal of primers and primer-dimers
• Innovative wash plate minimizes risk of cross-contamination
• Suitable for processing under vacuum and centrifugation
• Time-saving parallel clean-up of PCR products

Principle

Once the PCR reaction is complete, the reaction volume is adjusted and the Binding Solution is added. The samples are transferred to the Binding Plate where the PCR reaction is captured on the silica membrane. The bound PCR product is washed several times to remove primers, primer-dimers, salts, nucleotides and polymerases. Finally the purified PCR product is eluted and ready for immediate use in downstream applications.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Plasmid DNA Purification Overview

Extraction of macromolecules such as DNA, RNA, and protein is common in molecular biology research. The process of extraction and purification of nucleic acids has evolved from being a complex and la...
Keywords: Centrifugation, Chromatography, Cloning, DNA purification, Digestions, Genetic, Ion Exchange, Molecular biology, Nucleic acid denaturation, Nucleic acid purification, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transcription, transformation

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

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PCR9604 GenElute 96 Well PCR Clean-Up Kit, 4 x 96 well plate, sufficient for 4 96-well plate purifications

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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